Chikungunya One-Step SYBR Green I-based Real-Time RT-PCR
Chikungunya CHIKV) belongs to the Alphavirus genus of the Togaviridae family. It is an enveloped virus about 70-100 nm in virus. The virus is transmitted to humans by bites of infected female mosquitoes, especially Aedes albopictus and
Aedes aegypti, which are also the vectors of Dengue virus (DENV). Ae. aegypti are urban mosquitoes that maintain close association with humans and are therefore likely to be responsible for the large regional and global outbreaks. The
one-step SYBR Green I-based real-time RT-PCR assay that was developed by the institute could detect the presence of the virus in plasma samples. Samples from different hospitals are sent to the Central Laboratories for testing.
Sample requirement: Plasma (2ml-5ml EDTA)
The UPM - IBBC is organizing a Training Program for Laboratory Biosafety and Biosecurity Officers to commence in the second quarter of 2016. The main objective of the program is to develop a pool of local trainers who will undertake advocacy and continuing education on laboratory biosafety and biosecurity. At the end of the training, the trainers are expected to be competent in the following areas:
*Biosafety and biosecurity risk assessment *Laboratory vivarium Biosafety and Biosecurity *Biocontainment practices and procedures *Biocontainment facilities *Use of safety equipment *Incident response procedures *Infectious substance transport *Biosafety program management *Management of transgenic materials *Regulatory framework of transgenic materials
The training will entail at least ten modules, each of which with duration of two weeks, scheduled on July 18-29, 2016; September 18- October 1, 2016; and November 20- December 3, 2016.
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