High Throughput Simultaneous Detection of Waterborne Parasitosis from Household, Recreational and Environmental Water Samples Using Microarray Technology

Oligonucleutide microarrays have been used successfully for the detection of bacterial and viral pathogens. The distinct advantage of this detection approach is that it combines powerful DNA amplification strategies with subsequent hybridization to oligonucleotide probes specific for multiple target sites. This method allows for the simultaneous analysis of a larger number of genetic features in a single experiment. Thus, the amplification and hybridization approach produces a highly sensitive and specific platform with high-throughput capacity for pathogen detection and genotyping. Such methods would be ideally suited for the detection of waterborne protozoan parasites due to the increasing reliance upon genetic tests for identification and differentiation, the low concentration or number of organisms required to cause disease, and the often found presence of multiple protozoan species in a single environment or clinical sample. In this present study proposal, we want to demonstrate the use of oligonulcleotide microarray to simultaneously detecting and differentiating the primary waterborne protozoa pathogenic to humans, E. histolyca, G. lambdia, and C. parvumin the Philippines.

Biotech-Manila also focuses on the development of rapid diagnostic system that is "Low-cost and Low-technology driven" relevant to health and medical needs of the country.

Rapid POC testing for the diagnosis and determination of prognostic indicators of dengue hemorrhagic fever, multi-drug resistant organisms such as TB, hospital pathogens and other relevant emerging and re-emerging infections in the country and the world.


Ongoing Projects

3-D Targets: The UK Philippines Dengue Diagnostics and Drug Targets Research Consortium

HIV Drug Resistance Surveillance Library and Development of Molecular Diagnostics for Drug-Resistance Detection- Part 1: Surveillance of Mutations for Acquired Drug Resistance

Dengue: Climate, Evolution and Transmission

Improving the Molecular Diagnostics of Drug Resistant Tuberculosis

Responding to the Philippine HIV epidemic: an HIV drug resistance surveillance library and development of molecular diagnostics for drug-resistance detection:Part 2: Analysis and prevalence of pre-treatment drug resistance

News

CALL FOR APPLICATIONS FOR TRAINING FOR BIOSAFETY OFFICERS

The UPM - IBBC is organizing a Training Program for Laboratory Biosafety and Biosecurity Officers to commence in the second quarter of 2016. The main objective of the program is to develop a pool of local trainers who will undertake advocacy and continuing education on laboratory biosafety and biosecurity. At the end of the training, the trainers are expected to be competent in the following areas:

*Biosafety and biosecurity risk assessment *Laboratory vivarium Biosafety and Biosecurity *Biocontainment practices and procedures *Biocontainment facilities *Use of safety equipment *Incident response procedures *Infectious substance transport *Biosafety program management *Management of transgenic materials *Regulatory framework of transgenic materials

The training will entail at least ten modules, each of which with duration of two weeks, scheduled on July 18-29, 2016; September 18- October 1, 2016; and November 20- December 3, 2016.

For more information please click the download link: Download